People

Houming Zhang

Postdoctoral Fellows

Proteomic Manager

KAUST Bioscience Core Lab

Research Interests

Huoming is working to develop and apply advanced mass spectrometry based proteomics technologies such as iTRAQ, SILAC and label-free tools to address various questions in biomedical and life sciences. He focuses on the quantitative proteomics analysis of immune response of various immune cells (e.g. monocytes, macrophages, and T cells) to invading pathogens, which could lead to an in-depth understanding of mechanism and further the formulation of immune-diagnostic/-therapeutic strategies. He also applies proteomics approach to study protein expression in novel marine species.

Selected Publications

1.     Hao P#, Huoming Zhang#, Sze SK. Application of electrostatic repulsion hydrophilic interaction chromatography (ERLIC) to the characterization of proteome, glycoproteome and phosphoproteome Using Nano LC-MS/MS. Methods Mol Biol. NanoProteomics. (Invited for Book Chapter 23rd), in press. # equal contribution.

2.     Huoming Zhang, Wong YH, Wang H, Ravasi T, Qian PY. Quantitative proteomics identify molecular targets that are crucial in larval settlement and metamorphosis of Bugula neritina.  J Proteome Res 2011, 10(1): 349-60.

3.     Huoming Zhang, Zhao C, Li X, Zhu Y, Gan CS, Wang Y, Ravasi T, Qian PY, Wong SC, Sze SK. Study of monocyte membrane proteome perturbation during lipopolysaccharide-induced tolerance using iTRAQ-based quantitative proteomic approach. Proteomics. 2010, 10 (15), 2780-9

4.     Huoming Zhang, Guo T, Li X, Datta A, Yang J, Lim SK, Tam JP, Sze SK. Simultaneous Characterization of Glyco- and Phosphoproteomes of Mouse Brain Membrane Proteome with Electrostatic Repulsion Hydrophilic Interaction Chromatography. Molecular & Cellular Proteomics. 2010; 9(4):635-47.

5.     Park JE, Tan HS, Datta A, Lai RC, Huoming Zhang, Meng W, Lim SK, Sze SK. Hypoxia modulates tumor microenvironment to enhance angiogenic and metastastic potential by secretion of proteins and exosomes. Molecular & Cellular Proteomics. 2010; 9(6): 1085-1099.

6.     Zhao C#; Huoming Zhang#; Yeap WH; Gan CS; Sze SK; Kourilsky P, Wong SC. Identification of Novel Functional Differences in Monocyte Subsets Using Proteomic and Transcriptomic Methods. Journal of Proteome Research. 2009, 8(8), 4028-4038. #equal contribution.

7.     Wang H, Huoming Zhang, Wong YH, Voolstra C, Ravasi T, Vladimir BV, Qian PY, Rapid transcriptome and proteome profiling of a non-model marine invertebrate, Bugula neritinaProteomics. 2010, 10(16), 2972-81

8.     Meng W, Huoming Zhang, Guo T, Chhiti Pandey, Yi Zhu, Oi Lian Kon, Sze SK. One-step procedure for peptide extraction from in-gel digestion sample for mass spectrometric analysis. Analytical Chemistry, 2008, 80 (24): 9797–9805.

9.     Guo T, Gan CS, Huoming Zhang, Zhu Y, Oi Lian Kon, Sze SK, Hybridization of Pulsed-Q Dissociation and Collision-Activated Dissociation in Linear Ion Trap Mass Spectrometer for iTRAQ Quantitation, Journal of Proteome Research, 2008, 7(11), 4831-4840.

10. Gan CS, Guo T, Huoming Zhang, Lim SK and Sze Sk, A Comparative Study of Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC) versus SCX-IMAC-Based Methods for Phosphopeptide Isolation/Enrichment. Journal of Proteome Research, 2008. 7(11), 4869-4877.

11.Datta A, Park JE, Li X, Huoming Zhang, Ho ZS, Heese K, Lim SK, Tam JP, Sze SK. Phenotyping of an in Vitro Model of Ischemic Penumbra by iTRAQ-Based Shotgun Quantitative Proteomics. Journal of Proteome Research. 2010, 9 (1), 472–484.

12.  Huoming Zhang, Lin QS, Ponnusamy S, Kothandaraman N, Lim TK, Zhao CQ, Arijit B, Rauff M, Hew CL, Chung MCM, Joshi SB, Choolani M. Differential recovery of membrane proteins after extraction by aqueous methanol and trifluoroethanol. Proteomics. 2007. 7 (10), 1654-1663.

Education

2003-2007 PhD. Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
2001-2002 M.Sc. Molecular Engineering of Biological and Chemical System (MEBCS), Singapore-Massachusetts Institute of Technology Alliance (SMA)
1998-2001 M.Sc. Chemistry, Department of Polymer Chemistry and Physics, Zhejiang University, China.
1994-1998 B.Eng. Polymer Sciences, Department of Polymer Science and Engineering, Zhejiang University, China.